Pseudomonas fluorescens interaction. (A) Development of the interaction of Arabidopsis with P. fluorescens. (a), (b), and (c), photographs of 20-day-old Arabidopsis (Col-0) plantlets inoculated with P. fluorescens, the plantlets were photographed at (a) 72 h, (b) 96 h, and (c) 120 h post-inoculation. (d), (e) and (f), photographs of 20-day-old Arabidopsis (Col-0) control non-inoculated plantlets of (d) 72 h, (e) 96 h and (f) 120 h; (B) Expression analysis of the HR4 gene of Arabidopsis in interaction with beneficial bacterium P. fluorescens. Quantification of HR4 gene by qRT-PCR, expressed as relative mRNA level compared with a control condition, was calculated after normalization to the Arabidopsis actin 8. Control conditions or non-inoculated plantlets (light grey bars) and tester conditions or inoculated plantlets (dark bars) at 72, 96 and 120 hpi, respectively.