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. 2012 Jul 20;13(7):9110–9128. doi: 10.3390/ijms13079110

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Pseudomonas syringae interaction. (A) Development of the interaction of Arabidopsis with P. syringae pv. tomato DC3000. (a), (b), (c) and (d) photographs of 20-day-old Arabidopsis (Col-0) plantlets inoculated with P. syringae, the plantlets were photographed at 12 h (a), 24 h (b), 48 h (c) and 72 h (d) post-inoculation. (e), (f), (g), and (h), photographs of 20-day-old Arabidopsis (Col-0) control non-inoculated plantlets of 12 h (e), 24 h (f), 48 h (g) and 72 h (h); (B) Expression analysis of the HR4 gene of Arabidopsis in interaction with plant-pathogenic P. syringae. Quantification of the HR4 gene by qRT-PCR, expressed as relative mRNA level compared with a control condition, was calculated after normalization to the Arabidopsis actin 8. Control condition or non-inoculated plantlets (light grey bars) and tester conditions or inoculated plantlets (dark bars) at 12, 24, 48 and 72 hpi, respectively.