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. 2012 Jul 24;13(7):9363–9379. doi: 10.3390/ijms13079363

Figure 1.

Figure 1

Sensitivity of staurosporine (STS)-induced apoptotic volume decrease (AVD) and caspase activation to volume-sensitive outwardly rectifying Cl− channel (VSOR) blockers. The experiments with a VSOR blocker (200 μM 5-nitro-2-(3- phenylpropylamino)-benzoic acid (NPPB) or 100 μM phloretin) were performed in HeLa cells by adding either blocker to the bathing solution after pretreating the cells with either blocker for 1 h. (A, B) Time course of changes in the mean cell volume after stimulation with 4 μM STS in the absence (filled squares) or presence (open circles) of NPPB (A) or phloretin (B). Each symbol represents the relative mean cell volume at a given time (normalized by the mean cell volume at time zero), and each vertical bar represents the SEM value (n = 3–5). The mean cell volume (of around 3.3 pL) at time zero was not significantly different among experiments. * p < 0.05 versus the data in the absence of a VSOR blocker at a given time. † p < 0.05 between the data in the absence of a VSOR blocker at time zero and at a given time. (C) Caspase-3/7 activity before (Control: open column) or after 4-h stimulation with 4 μM STS in the absence (filled column) or presence of NPPB or phloretin (shadowed columns). Each column represents the relative mean value (normalized by the control value) with SEM (vertical bar) (n = 18). * p < 0.05 between two data designated.