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. 2012 Jul 24;13(7):9363–9379. doi: 10.3390/ijms13079363

Figure 5.

Figure 5

Effects of siRNA-mediated knockdown of ASK1 on the ASK1 mRNA level, STS-induced caspase activation and AVD in HeLa cells. (A) Down-regulation of ASK1 mRNA expression by 2-day treatment with ASK1 siRNA but not by that with negative control siRNA. Each column represents the relative mean ratio of ASK1 mRNA to β-actin mRNA (normalized by the non-treated control value) with SEM (vertical bar) (n = 3). (B) Caspase-3/7 activity after 6-h stimulation with 4 μM STS in the cells transfected with ASK1 siRNA and negative control siRNA. Each column represents the relative mean value (normalized by the mean value in the absence of STS) with SEM (vertical bar) (n = 4). * p < 0.05 between two data designated. (C) Time course of changes in the mean cell volume after stimulation with 4 μM STS in the cells transfected with ASK1 siRNA (open circles) or negative control siRNA (filled squares). Each symbol represents the relative mean value (normalized by the mean cell volume at time zero) with SEM (vertical bar) (n = 6–7). The data treated with ASK1 siRNA were not significantly different from those treated with negative control siRNA at any time points tested. † p < 0.05 between the negative control data at time zero and at a given time.