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. 2012 Jul 24;13(7):9363–9379. doi: 10.3390/ijms13079363

Figure 7.

Figure 7

NPPB insensitivity of p38 phosphorylation induced by sustained osmotic cell shrinkage in HeLa cells. (A) Western blot analysis for changes in the levels of P-38 and p38 after incubation in hypertonic (600 mOsmol/kg-H2O) solution containing 100 μM FFA in the absence (−) or presence (+) of 200 μM NPPB for 30 min. (B) Ratio of P-p38 to p38 after incubation in 600 mOsmol/kg-H2O (mOsm) solution containing 100 μM FFA in the absence or presence of 200 μM NPPB. Each symbol represents the relative mean value (normalized by the control value at 300 mOsm in the absence of FFA) with SEM (vertical bar) (n = 5). * p < 0.05 versus the data without NPPB.