Fig 2.

The Hcp2 protein is secreted by P. syringae pv. tomato DC3000. Protein samples were prepared from supernatants and cell pellets of fresh overnight cultures in KB. β-ME was added to the samples on the left and omitted from the samples on the right. The proteins were detected by using anti-Hcp1 antiserum and an anti-rabbit monoclonal antibody conjugated with alkaline phosphatase, which degrades the Nitro Blue Tetrazolium–5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (BCIP) substrate, producing the color violet. The lower panels show immunodetection of Hcp from the cell fractions of the same samples with and without β-ME. Only the monomeric form could be detected in the cell fractions treated with β-ME. St, standard proteins; WT, P. syringae pv. tomato DC3000 parent strain; D1, Δhcp1 deletion mutant; D2, Δhcp2 deletion mutant; DD, Δhcp1 Δhcp2 double deletion mutant.