Table 2.
Primers used in PCRs to create plasmid constructs for generation of in-frame chromosomal deletions in P. syringae pv. tomato DC3000
| Primer | Sequence (5′→3′) | Feature of underlined sequence |
|---|---|---|
| Pto_2539mt-1 | CCGAATTCGCGGAAAAACGCCTTGGAAAGG | EcoRI site |
| Pto_2539mt-2 | GCGAGATTATTTCGGACTGCATTGGTTTCTC CTGGAAGGTTA | Region complementary to primer Pto_2539mt-3 |
| Pto_2539mt-3 | ATGCAGTCCGAAATAATCTCGC | |
| Pto_2539mt-4 | CCAAGCTTTTATCCA TTGCGCGACCTCT | HindIII site |
| Pto_5435mt-1 | CCGGATCCTTCCAGCTCGACCAAGAGATC | BamHI site |
| Pto_5435mt-2 | TGATTGGCCGGCGCGAACATGGAGGTGCTCCTTGCTGGAT | Region complementary to primer Pto_5435mt-3 |
| Pto_5435mt-3 | ATGTTCGCGCCGGCCAATCA | |
| Pto_5435mt-4 | CCTCTAGATCAGATCGCACACGCACCGT | XbaI site |
| Pto_2554mt-1 | CCGGATCCATGAATTCGCACAAGGTTATCTG | BamHI site |
| Pto_2554mt-2 | CTAAAGATCCCGTGACATCAGACTTTTCTCTTGTCAGGATCGAG | Region complementary to Pto_2554mt-3 |
| Pto_2554mt-3 | TGATGTCACGGGATCTTTAGAACCG | |
| Pto_2554mt-4 | CCTCTAGATCAACCCGCGGTACTGGTCG | XbaI site |
| Pto_5418mt-1 | CCTCTAGAACACCCATGAACCGCTCGCC | XbaI site |
| Pto_5418mt-2 | CAAGGGCTGGCTACCAGCATTTACGGCTGTGACTGCGGCTGGAC | Region complementary to Pto_5418mt-3 |
| Pto_5418mt-3 | ATGCTGGTAGCCAGCCCTTGGCGC | |
| Pto_5418mt-4 | CCAAGCTTGCTGCGCCGACATCTGCTGA | HindIII site |