Fig 5.

Organization of the ohr-ohrR locus in Brucella abortus 2308. (A) Primer extension analysis of the ohr mRNA was employed to determine the start site of transcription. The primer extension product, along with the corresponding DNA sequencing reaction products, was separated on 6% denaturing polyacrylamide gels and visualized by autoradiography. (B) Schematic representation of the ohr-ohrR locus. The transcriptional start sites of the ohr and ohrR mRNAs are denoted with an arrow, and the −10 and −35 elements of the promoters are shown. The region of the ohr-ohrR intergenic region that is bound by OhrR is underlined.