Table 4.
Oligonucleotides for binding site studies
| Binding oligonucleotide | Sequence (5′–3′)a | Reference |
|---|---|---|
| Pemm1 MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | 1 |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 G9A MBS 49-mer | AGCATTTAAGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATTCAGTTTTTCCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 G10A MBS 49-mer | AGCATTTAGATCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCTAGTTTTTCCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 T11C MBS 49-mer | AGCATTTAGGCCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCGGTTTTTCCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 C12A MBS 49-mer | AGCATTTAGGTAAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCATTTTTTCCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 A13C MBS 49-mer | AGCATTTAGGTCCAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGGTTTTCCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 G18A MBS 49-mer | AGCATTTAGGTCAAAAAAGTGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTTCACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 G19A MBS 49-mer | AGCATTTAGGTCAAAAAGATGGCAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCTACCGTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 C23A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGAAAAAGCTAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCTTTTTCGATTTTTTCGACCAGAAATGG | ||
| Pemm1 C29A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGATAAAAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCTATTTTTTCGACCAGAAATGG | ||
| Pemm1 A33C MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAACAAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTGTTTCGACCAGAAATGG | ||
| Pemm1 A34C MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAACAAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTGTTCGACCAGAAATGG | ||
| Pemm1 A35C MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAACAGCTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTGTCGACCAGAAATGG | ||
| Pemm1 G37A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAACTGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTTGACCAGAAATGG | ||
| Pemm1 C38A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGATGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCTACCAGAAATGG | ||
| Pemm1 T39C MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCCGGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGGCCAGAAATGG | ||
| Pemm1 G40A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTAGTCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGATCAGAAATGG | ||
| Pemm1 G41A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTGATCTTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGACTAGAAATGG | ||
| Pemm1 C43A MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTATTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGACCATAAATGG | ||
| Pemm1 T44C MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCCTTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGACCAGGAATGG | ||
| Pemm1 T45C MBS 49-mer | AGCATTTAGGTCAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTCTCTACC | This study |
| TCGTAAATCCAGTTTTTCCACCGTTTTCGATTTTTTCGACCAGAGATGG | ||
| Pemm1 C12/43A MBS 49-mer | AGCATTTAGGTAAAAAAGGTGGCAAAAGCTAAAAAAGCTGGTATTTACC | This study |
| TCGTAAATCCATTTTTTCCACCGTTTTCGATTTTTTCGACCATAAATGG | ||
| PscpA1 MBS 49-mer | GGATAAGGAGGTCACAAACTAAGCAACTCTTAAAAAGCTGACCTTTACT | This study |
| CCTATTCCTCCAGTGTTTGATTCGTTGAGAATTTTTCGACTGGAAATGA | ||
| PscpA1 C12A MBS 49-mer | GGATAAGGAGGTAACAAACTAAGCAACTCTTAAAAAGCTGACCTTTACT | This study |
| CCTATTCCTCCATTGTTTGATTCGTTGAGAATTTTTCGACTGGAAATGA | ||
| PscpA1 G40A MBS 49-mer | GGATAAGGAGGTCACAAACTAAGCAACTCTTAAAAAGCTAACCTTTACT | This study |
| CCTATTCCTCCAGTGTTTGATTCGTTGAGAATTTTTCGATTGGAAATGA | ||
| PscpA1 C43A MBS 49-mer | GGATAAGGAGGTCACAAACTAAGCAACTCTTAAAAAGCTGACATTTACT | This study |
| CCTATTCCTCCAGTGTTTGATTCGTTGAGAATTTTTCGACTGTAAATGA | ||
| PscpA1 C12/43A MBS 49-mer | GGATAAGGAGGTAACAAACTAAGCAACTCTTAAAAAGCTGACATTTACT | This study |
| CCTATTCCTCCATTGTTTGATTCGTTGAGAATTTTTCGACTGTAAATGA | ||
| Psic1 MBS 49-mer | GTAAGGAGAGGTCACAAACTAAACAACTCTTAAAAAGCTGACCTTTACT | This study |
| CATTCCTCTCCAGTGTTTGATTTGTTGAGAATTTTTCGACTGGAAATGA | ||
| Psic1 C12A MBS 49-mer | GTAAGGAGAGGTAACAAACTAAACAACTCTTAAAAAGCTGACCTTTACT | This study |
| CATTCCTCTCCATTGTTTGATTTGTTGAGAATTTTTCGACTGGAAATGA | ||
| Psic1 G40A MBS 49-mer | GTAAGGAGAGGTCACAAACTAAACAACTCTTAAAAAGCTAACCTTTACT | This study |
| CATTCCTCTCCAGTGTTTGATTTGTTGAGAATTTTTCGATTGGAAATGA | ||
| Psic1 C43A MBS 49-mer | GTAAGGAGAGGTCACAAACTAAACAACTCTTAAAAAGCTGACATTTACT | This study |
| CATTCCTCTCCAGTGTTTGATTTGTTGAGAATTTTTCGACTGTAAATGA | ||
| Psic1 C12/43A MBS 49-mer | GTAAGGAGAGGTAACAAACTAAACAACTCTTAAAAAGCTGACATTTACT | This study |
| CATTCCTCTCCATTGTTTGATTTGTTGAGAATTTTTCGACTGTAAATGA | ||
| MBS Random 49-mer | TTTAGAAACAAAGGCATCAGTCGACCTGAAGCTATTTAGAAAAAGGGTC | This study |
| AAATCTTTGTTTCCGTAGTCACGTGGACTTCGATAAATCTTTTTCCCAG |
a
Mutated nucleotides are shown in bold underlined type.