Fig 6.

Distinct pglA alleles are associated with variable levels of microheterogeneity. Immunoblotting of whole-cell lysates from mutant strains of FA1090 and N400, carrying pglA alleles from different N. gonorrhoeae, N. meningitidis, and N. lactamica strains, with glycan-specific monoclonal antibodies (npg1, npg2, and npg3). Note that the pglA genes from N400, FA1090, Z2491, and MC58 are in the phase-on configuration, while those from 8013, ST3787, ST640, and FAM18 are in the phase-off configuration and are marked with asterisks (the alignment is shown in Fig. S1 in the supplemental material). N400-derived strains were KS100, KS462, KS483, KS484, KS742, KS743, KS744, KS477, KS478, and KS745. FA1090-derived strains were KS300, KS463, KS482, KS481, KS763, KS764, KS765, KS479, KS480, and KS766.