Fig 7.
Quantification of the variable levels of microheterogeneity. (A) The poly(G) tracts within the pglA ORFs from N. gonorrhoeae strains (N400, FA1090), N. meningitidis strains (8013, FAM18), and N. lactamica strains (ST640, ST3787) are shown with the corresponding amino acids above (gray). The nucleotide numbering is according to pglAN400. (B) Immunoblotting of whole-cell lysates from mutant strains of N400 carrying pglA alleles from different N. gonorrhoeae, N. meningitidis, and N. lactamica strains with the npg2 glycan-specific monoclonal antibody. The amount of disaccharide glycan in N400 carrying different pglA alleles was compared to the level of disaccharide glycan in admixtures of N400 wild type (KS100) and N400 pglA::ermC-rpsL (KS462). Other strains used were KS744, KS745, KS478, KS477, and KS483. Note that the pglA genes from 8013, ST3787, ST640, and FAM18 are in the phase-off configuration.