Fig 8.
Polymorphic alleles of pglA and pglH influence microheterogeneity. Immunoblotting of whole-cell lysates from mutant strains of FA1090 carrying different pglA and pglH alleles with the glycan-specific monoclonal (npg1, npg2, and npg3) and polyclonal (pDAb2, which recognizes diNAcBac-Glc) antibodies. +, wild-type pglA and pglH alleles; −, null alleles pglA::kan and pglH::ermC-rpsL; +, hypermorphic alleles pglHFA1090 H371R and pglAN400. Strains with the most active allele of pglH had increased diNAcBac-Glc in backgrounds without PglA or with less active PglA (compare the fourth and sixth lanes with the second lane). Strains with most active allele of pglA had less diNAcbac-Glc, and instead, more trisaccharide was detected (seventh lane). Strains used were KS300, KS769, KS422, KS459, KS423, KS460, and KS770.