Table 1.
Strains used in this study
| Strain | Parental strain | Relevant genotype | Reference or source |
|---|---|---|---|
| N. gonorrhoeae N400 strains | |||
| KS100 (N400) | VD300 | recA6a | 25 |
| KS461 | KS100 | recA6 pglEon pglA::ermC-rpsL | This study |
| KS462 | KS100 | recA6 pglA::ermC-rpsL | This study |
| KS488 | KS462 | recA6 pglA5′ FA1090 | This study |
| KS489 | KS462 | recA6 pglA5′ N400 | This study |
| KS477 | KS462 | recA6 pglAST3787 | This study |
| KS478 | KS462 | recA6 pglAST640 | This study |
| KS483 | KS462 | recA6 pglAFA1090 | This study |
| KS484 | KS462 | recA6 pglAN400 | This study |
| KS742 | KS462 | recA6 pglAZ2491 | This study |
| KS743 | KS462 | recA6 pglAMC58 | This study |
| KS744 | KS462 | recA6 pglA8013 | This study |
| KS745 | KS462 | recA6 pglAFAM18 | This study |
| KS473 | KS462 | recA6 pglAGGGG | This study |
| KS474 | KS462 | recA6 pglAGGGG, E275A | This study |
| KS492 | KS462 | recA6 pglAG350A | This study |
| KS493 | KS461 | recA6 pglEon pglAG350A | This study |
| KS495 | KS462 | recA6 pglAE275A | This study |
| KS101 | VD300 | pilEind | 29 |
| KS122 | KS101 | pilEind pglA::kan | 27 |
| KS773 | KS122 | pilEind pglA::kan lct::pglAN400 | This study |
| KS771 | KS122 | pilEind pglA::kan lct::pglAFA1090 | This study |
| KS772 | KS122 | pilEind pglA::kan lct::pglAST3787 | This study |
| N. gonorrhoeae FA1090 strains | |||
| KS300 | FA1090 | recA6a | 25 |
| KS463 | KS300 | recA6 pglA::ermC-rpsL | This study |
| KS464 | KS300 | recA6 pglEoff pglA::ermC-rpsL | This study |
| KS465 | KS463 | recA6 pglAPRGSG | This study |
| KS466 | KS463 | recA6 pglAPRGSGDLEQPA | This study |
| KS467 | KS463 | recA6 pglAQPA, A275E | This study |
| KS468 | KS463 | recA6 pglAPRGSG, A275E | This study |
| KS469 | KS463 | recA6 pglAQPA | This study |
| KS470 | KS463 | recA6 pglAGSG, A275E | This study |
| KS471 | KS463 | recA6 pglAPRGSGDLEQPA, A275E | This study |
| KS472 | KS463 | recA6 pglAGSG | This study |
| KS475 | KS463 | recA6 pglAA146T | This study |
| KS476 | KS463 | recA6 pglAPRGSGDLEQPA, A275E, A146T | This study |
| KS479 | KS463 | recA6 pglAST3787 | This study |
| KS480 | KS463 | recA6 pglAST640 | This study |
| KS481 | KS463 | recA6 pglAN400 | This study |
| KS482 | KS463 | recA6 pglAFA1090 | This study |
| KS763 | KS463 | recA6 pglAZ2491 | This study |
| KS764 | KS463 | recA6 pglAMC58 | This study |
| KS765 | KS463 | recA6 pglA8013 | This study |
| KS766 | KS463 | recA6 pglAFAM18 | This study |
| KS486 | KS463 | recA6 pglA5′ N400 | This study |
| KS487 | KS463 | recA6 pglA5′ FA1090 | This study |
| KS490 | KS463 | recA6 pglAA350G | This study |
| KS491 | KS463 | recA6 pglAPRGSGDLEQPA, A275E, A146T, A350G | This study |
| KS494 | KS464 | recA6 pglEoff, pglAA350G | This study |
| KS496 | KS463 | recA6 pglAA275E | This study |
| KS769 | KS300 | recA6 pglA::kan | This study |
| KS422 | KS300 | recA6 pglH::ermC-rpsL | 8 |
| KS459 | KS422 | recA6 pglHH371R | 8 |
| KS423 | KS422 | recA6 pglA pglH | 8 |
| KS460 | KS423 | recA6 pglA::kan pglHH371R | 8 |
| KS770 | KS460 | recA6 pglAN400 pglHH371R | This study |
a
recA6 is an IPTG-inducible allele of recA.