. 2012 Sep;194(18):4894–4903. doi: 10.1128/JB.00782-12

Table 2.

Cma sensitivities of E. coli strains transformed with plasmids carrying cbrA

Strain	Diam of lysis zone (cm) with 10-fold dilution series
Strain	10⁰	10¹	10²	10³	10⁴	10⁵	10⁶
AB2847	2	1.6	1.4	1.05	0.9	0.7	0.8^a
AB2847(pKH35)	1.7^a	1.5^a	1.1^a	0.9^a
AB2847^b	1.9	1.6	1.4	1.2	0.8	0.7^a
AB2847(pSH150), uninduced	1.7^a	1.3^a	1.0^a	0.9^a
AB2847(pSH150), induced	0	0	0	0
BW25113	ND	1.7	1.5	1.3	1.0	0.9^a
JW5631 ΔcbrA	ND	1.8	1.5	1.3	1.0	0.8^a

Turbid lysis zone.

In this and the four following experiments, a less active Cma solution was used. An aliquot of a 10-fold dilution series of the purified Cma stock solution was spotted on an LB agar plate seeded with one of the strains to be tested. Cells carrying pSH150 also carried pHK569, which is a derivative of pGP1-2 (44) and encodes the T7 polymerase under the control of the temperature-sensitive λcI857 repressor. ND, not determined. The data were fully reproducible in several experiments.