Table 4.
Strain | Medium | Growth | Activity (U) |
---|---|---|---|
BW25113(pKH55) | M9 | Aerobic | 16.2 |
JW4361 ΔcreB(pKH55) | M9 | Aerobic | 3.6 |
JW5631 ΔcbrA(pKH55) | M9 | Aerobic | 14.4 |
BW25113(pKH55) | M9 | Anaerobic | 14.5 |
JW4361 ΔcreB(pKH55) | M9 | Anaerobic | 1.6 |
JW5631 ΔcbrA(pKH55) | M9 | Anaerobic | 13.3 |
BW25113(pKH55) | LB | Aerobic | 2.1 |
JW4361 ΔcreB(pKH55) | LB | Aerobic | 0.7 |
JW5631 ΔcbrA(pKH55) | LB | Aerobic | 1.5 |
Plasmid pKH55 encodes the pcbrA-lacZ operon fusion under the control of CreB. The values varied ±5% in repeated experiments.