Fig 6.

Rob does not directly bind salicylate. Measurements were made using a VP-ITC (MicroCal) calorimeter with purified Rob (10 μM) and 5 mM 2,2-dipyridyl (A), 5 mM salicylate (B), and 10 mM decanoate (C). Data were collected and analyzed using the Origin-based MicroCal software suite. We also tested whether a buffer-only control with 5 mM 2,2-dipyridyl would also yield an enthalpic change due to metal chelation. While the buffer-only control yielded an enthalpic change, it was appreciably less than that with Rob present (data not shown).