Fig 7.

Thin-layer chromatography analysis of cellulose hydrolysis products. 1% CM-cellulose solutions were incubated at 80°C with SSO1354 enzyme and with a 1:1 (enzyme units) mixture of SSO1354 and S. solfataricus β-glycosidase. Aliquots of the reaction mixtures with SSO1354 (lanes 1 to 4), with SSO1354 and β-glycosidase (lane 5), and without (lanes B₀ and B₁₆) enzyme(s) were withdrawn at different time points and spotted onto silica plates. Reaction products were separated with a mixture of ethyl acetate, H₂O, acetic acid, isopropanol, and formic acid (25:15:10:5:1) and visualized with α-naphthol after incubation for 5 min at 120°C. Lane M, mix of cello-oligomers (3 μl of a solution with a concentration of 5 mM [each] oligomer); B₀ and B₁₆, cellulose solutions in buffer without enzyme(s) after no and 16 h of incubation, respectively; 1, 2 h of incubation, 2, 4 h of incubation, 3,8 h of incubation, 4, 16 h of incubation, 5, 16 h of incubation with the mixture of the two enzymes.