Fig 2.

DnaD does not affect nucleotide exchange by DnaA. The amount of [¹⁴C]ADP (A) or [³²P]ATP (B) bound to DnaA (300 nM) at various times after addition of unlabeled ATP (2 mM) at 37°C was measured by filter binding in the absence (open circles) and presence (filled squares) of DnaD-His₆ (600 nM). Data are averages of triplicates ± standard errors and are normalized to the starting amount of radioactivity in the absence of unlabeled ATP.