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Effects of proline 613 substitutions in PilQNm and of the corresponding P562L substitution in PilQPa on autoassembly in vitro. (A) Total in vitro synthesis reactions producing wild-type PilQNm or its variants were analyzed by SDS-PAGE and immunoblotting with N. meningitidis PilQ antisera. (B) Total in vitro synthesis reactions producing wild-type PilQPa and PilQPa(P562L) from P. aeruginosa were analyzed by SDS-PAGE and immunoblotting with PilQPa antisera. Mu, PilQ multimer.
