Fig 2.

Deletion of any of the known Hfx. volcanii agl genes interferes with swimming motility. (A) Hfx. volcanii H53 (wild type [wt]) and Δagl strains were stab inoculated into 0.3% agar in complex medium (MGM) and incubated at 45°C for 5 days. (B) For the Hfx. volcanii ΔaglE strain, a motility halo is clearly visible after 8 days of incubation. A plate lacking the wild-type stab was used for this longer incubation, as the wild-type motility halo would have migrated across much of the plate at this time (horizontal bar, 1 cm). All inoculations were repeated at least three times for the verification of the phenotype.