Plasmids |
|
|
pTA131 |
Ampr; pBluescript II with BamHI-XbaI fragment from pGB70 harboring pfdx-pyrE2
|
7 |
pRV1-ptna
|
Ampr Novr; inducible ptna promoter |
24 |
pTA963 |
Ampr, pyrE2 and hdrB markers, inducible ptna promoter |
5 |
pUC19 |
Ampr
|
45 |
pMT7 |
pRV1-ptna carrying flgA1-His |
This study |
pMT8 |
pRV1-ptna carrying flgA2-His |
This study |
pMT9 |
pTA963 carrying flgA1-His |
This study |
pJY1 |
pTA131 carrying chromosomal flanking regions of flgA1
|
This study |
pST1 |
pTA963 carrying flgA1-His with an N70Q mutation |
This study |
pST2 |
pTA963 carrying flgA1-His with an N115Q mutation |
This study |
pST3 |
pTA963 carrying flgA1-His with an N172Q mutation |
This study |
pST4 |
pTA963 carrying flgA1-His with N70Q, N115Q and N172Q mutations |
This study |
Strains |
|
|
DH5α |
E. coli F− ϕ80dlacZΔM15 (lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) phoA supE44 thi-1 gyrA96 relA1
|
Invitrogen |
DL739 |
E. coli MC4100 recA
dam-13::Tn9
|
10 |
H53 |
Hfx. volcanii ΔpyrE2 Δtrp
|
7 |
MT2 |
H53 ΔflgA1 ΔflgA2
|
39 |
MT13 |
H53 containing pTA963 |
This study |
MT14 |
H53 ΔflgA1
|
This study |
MT15 |
MT14 containing pTA963 |
This study |
MT16 |
MT14 containing pMT9 |
This study |
ΔaglB
|
H53 ΔaglB
|
1 |
ΔaglJ
|
H53 ΔaglJ
|
33 |
ΔaglG
|
H53 ΔaglG
|
46 |
ΔaglM
|
H53 ΔaglM
|
47 |
ΔaglF
|
H53 ΔaglF
|
46 |
ΔaglI
|
H53 ΔaglI
|
46 |
ΔaglE
|
H53 ΔaglE
|
3 |
ΔaglP
|
H53 ΔaglP
|
26 |
ΔaglD
|
H53 ΔaglD
|
1 |
JY1 |
H53 ΔaglB containing pMT7 |
This study |
JY2 |
H53 ΔaglJ containing pMT7 |
This study |
JY3 |
H53 ΔaglG containing pMT7 |
This study |
JY4 |
H53 ΔaglM containing pMT7 |
This study |
JY5 |
H53 ΔaglF containing pMT7 |
This study |
JY6 |
H53 ΔaglI containing pMT7 |
This study |
JY7 |
H53 ΔaglE containing pMT7 |
This study |
JY8 |
H53 ΔaglP containing pMT7 |
This study |
JY9 |
H53 ΔaglD containing pMT7 |
This study |
JY10 |
H53 ΔaglB containing pMT8 |
This study |
JY11 |
H53 ΔaglJ containing pMT8 |
This study |
JY12 |
H53 ΔaglG containing pMT8 |
This study |
JY13 |
H53 ΔaglM containing pMT8 |
This study |
JY14 |
H53 ΔaglF containing pMT8 |
This study |
JY15 |
H53 ΔaglI containing pMT8 |
This study |
JY16 |
H53 ΔaglE containing pMT8 |
This study |
JY17 |
H53 ΔaglP containing pMT8 |
This study |
JY18 |
H53 ΔaglD containing pMT8 |
This study |
ST1 |
MT14 containing pST1 |
This study |
ST2 |
MT14 containing pST2 |
This study |
ST3 |
MT14 containing pST3 |
This study |
ST4 |
MT14 containing pST4 |
This study |