Table 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Plasmids | ||
| pTA131 | Ampr; pBluescript II with BamHI-XbaI fragment from pGB70 harboring pfdx-pyrE2 | 7 |
| pRV1-ptna | Ampr Novr; inducible ptna promoter | 24 |
| pTA963 | Ampr, pyrE2 and hdrB markers, inducible ptna promoter | 5 |
| pUC19 | Ampr | 45 |
| pMT7 | pRV1-ptna carrying flgA1-His | This study |
| pMT8 | pRV1-ptna carrying flgA2-His | This study |
| pMT9 | pTA963 carrying flgA1-His | This study |
| pJY1 | pTA131 carrying chromosomal flanking regions of flgA1 | This study |
| pST1 | pTA963 carrying flgA1-His with an N70Q mutation | This study |
| pST2 | pTA963 carrying flgA1-His with an N115Q mutation | This study |
| pST3 | pTA963 carrying flgA1-His with an N172Q mutation | This study |
| pST4 | pTA963 carrying flgA1-His with N70Q, N115Q and N172Q mutations | This study |
| Strains | ||
| DH5α | E. coli F− ϕ80dlacZΔM15 (lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) phoA supE44 thi-1 gyrA96 relA1 | Invitrogen |
| DL739 | E. coli MC4100 recA dam-13::Tn9 | 10 |
| H53 | Hfx. volcanii ΔpyrE2 Δtrp | 7 |
| MT2 | H53 ΔflgA1 ΔflgA2 | 39 |
| MT13 | H53 containing pTA963 | This study |
| MT14 | H53 ΔflgA1 | This study |
| MT15 | MT14 containing pTA963 | This study |
| MT16 | MT14 containing pMT9 | This study |
| ΔaglB | H53 ΔaglB | 1 |
| ΔaglJ | H53 ΔaglJ | 33 |
| ΔaglG | H53 ΔaglG | 46 |
| ΔaglM | H53 ΔaglM | 47 |
| ΔaglF | H53 ΔaglF | 46 |
| ΔaglI | H53 ΔaglI | 46 |
| ΔaglE | H53 ΔaglE | 3 |
| ΔaglP | H53 ΔaglP | 26 |
| ΔaglD | H53 ΔaglD | 1 |
| JY1 | H53 ΔaglB containing pMT7 | This study |
| JY2 | H53 ΔaglJ containing pMT7 | This study |
| JY3 | H53 ΔaglG containing pMT7 | This study |
| JY4 | H53 ΔaglM containing pMT7 | This study |
| JY5 | H53 ΔaglF containing pMT7 | This study |
| JY6 | H53 ΔaglI containing pMT7 | This study |
| JY7 | H53 ΔaglE containing pMT7 | This study |
| JY8 | H53 ΔaglP containing pMT7 | This study |
| JY9 | H53 ΔaglD containing pMT7 | This study |
| JY10 | H53 ΔaglB containing pMT8 | This study |
| JY11 | H53 ΔaglJ containing pMT8 | This study |
| JY12 | H53 ΔaglG containing pMT8 | This study |
| JY13 | H53 ΔaglM containing pMT8 | This study |
| JY14 | H53 ΔaglF containing pMT8 | This study |
| JY15 | H53 ΔaglI containing pMT8 | This study |
| JY16 | H53 ΔaglE containing pMT8 | This study |
| JY17 | H53 ΔaglP containing pMT8 | This study |
| JY18 | H53 ΔaglD containing pMT8 | This study |
| ST1 | MT14 containing pST1 | This study |
| ST2 | MT14 containing pST2 | This study |
| ST3 | MT14 containing pST3 | This study |
| ST4 | MT14 containing pST4 | This study |