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(A) Scheme for glutathione-dependent peroxidase assay. (B) Reaction traces plus 5 μM LmTDPX1 or (C) plus bovine GSH peroxidase. (D) Scheme for tryparedoxin-dependent peroxidase assay. (E) Reaction traces plus 5 μM LmTDPX1 or (F) bovine GSH peroxidase. All reactions were started with the addition of 300 μM H2O2 (arrow). Symbols: without enzyme (open circles); plus enzyme (closed circles). For further details see “experimental procedures”.
