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. Author manuscript; available in PMC: 2012 Aug 29.
Published in final edited form as: FEBS J. 2007 Oct 8;274(21):5643–5658. doi: 10.1111/j.1742-4658.2007.06087.x

Figure 4. Kinetic analysis of TDPX1.

Figure 4

Representative data are shown for cumene hydroperoxide and kinetic parameters for this and other substrates are reported in Table 2. TryX was fixed at 0.5 μM (open circle), 1 μM (filled circle), 2 μM (open square), 3 μM (filled square) or 5 μM (open triangle) and cumene hydroperoxide concentrations were varied (50-1000 μM). Initial velocities were determined and globally fitted by non-linear regression to an equation describing a ping-pong mechanism (see “experimental procedures for further details). (A) Double reciprocal transformation of primary data showing the best fit. (B) Secondary plot of the intercepts of the primary plot A versus the reciprocal TryX concentrations.