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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: Cell. 2012 Jul 20;150(2):291–303. doi: 10.1016/j.cell.2012.05.042

Figure 4. Structural transition of RfaH-CTD in full-length RfaH.

Figure 4

(A, B) Overlay of [1H,15N]-HSQC spectra of 15N-RfaH-E48S, 45 μM, black, 15N-RfaH, 189 μM, cyan, and 15N-RfaH-CTD, 344 μM, red. Full spectra (A), and enlargement of one region (B). Signals from the CTD in full-length RfaH (α-helical form), cyan, from the isolated CTD (β-barrel form), red.

(C) Overlay of [1H,15N]-HSQC spectra of 15N-RfaH(NTD-TEV-CTD), 138 μM, black, 15N-RfaH, 189 μM, cyan, and 15N-RfaH(NTD-TEV-CTD) after incubation with 1.75 μM TEV protease for 42 hours, 127 μM, red.

(D) Overlay of [1H,15N]-HSQC spectra of 15N-RfaH(NTD-TEV-CTD) 127 μM, red. after incubation with 1.75 μM TEV protease for 42 hours from (C) and 15N-RfaH-CTD, 344 μM, black; signals of the isolated CTD in the β-barrel conformation, red.

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