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. Author manuscript; available in PMC: 2012 Aug 29.
Published in final edited form as: Cell. 2007 Mar 23;128(6):1105–1118. doi: 10.1016/j.cell.2007.01.038

Figure 2. Cell-Cycle Arrest Increases Translation Mediated by the TNFα ARE.

Figure 2

(A) Eighteen hours after transfection as in Figure 1, HEK293 cells were treated with aphidicolin dissolved in DMSO or with DMSO alone for 36 hr before measuring luciferase activities and mRNA levels by RPA. The values in panels (A) and (B) are averages from at least three transfections ± SD.

(B) After transfection, cells were grown for 66 hr to saturation. Then, one set was replated in fresh media; six hours later, luciferase and mRNA levels were assessed. Cell-cycle arrest was validated by western blotting for Ki67 (Figure S3 and Table S3).