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. 2012 Aug 9;36(10):909–915. doi: 10.1042/CBI20110562

Figure 3. ALP staining to detect ALP mRNA of OB in OB-HUVEC co-culture.

Figure 3

(A) Time-dependent ALP mRNA in indirect MG-63-HUVEC co-culture compared with MG-63 cells alone. (B) Time-dependent ALP mRNA in indirect MOB-HUVEC co-culture compared with MOB. (C) Effect of CGRP on ALP mRNA in monoculture of MG-63 or HUVEC, and direct or indirect MG-63-HUVEC co-culture compared with respective control groups after 48 h. (D) Effect of CGRP on ALP mRNA in monoculture of MOB or HUVEC, and direct or indirect MOB-HUVEC co-culture compared with respective control groups after 48 h. ALP mRNA in OB was detected by quantitative PCR. RQ% of target mRNA was calculated and expressed as the means±S.E.M., n = 6; *P<0.05, **P<0.01, compared with control groups.