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. 2012 Aug 9;36(10):909–915. doi: 10.1042/CBI20110562

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Time-dependent collagen/mRNA in indirect MG-63-HUVEC co-culture compared with MG-63 alone. (B) Time-dependent collagen/mRNA in indirect MOB-HUVEC co-culture compared with MOB. (C). Effect of CGRP on collagen/mRNA in monoculture of MG-63 or HUVEC and direct or indirect MG-63-HUVEC co-culture compared with respective control groups at 48 h. (D) Effect of CGRP on collagen/mRNA in monoculture of MOB or HUVEC and direct or indirect MOB-HUVEC co-culture compared with respective control groups after 48 h. Collagen/mRNA in OB were detected by quantitative PCR. RQ% of target mRNA was calculated and expressed as the means±S.E.M., n = 6; *P<0.05, **P<0.01, compared with control groups.