Table 3.
Reported expression of LEC markers by macrophage-derived lymphatic endothelial cell progenitors (M-LECP).
| Gene name | Comments | Detection method | Ref. |
|---|---|---|---|
| VEGFR-3 | Expressed by TG-stimulated peritoneal macrophages in culture | RT-PCR | [16] |
| Detected in TG-induced peritoneal macrophages in culture | RT-qPCR | [19] | |
| Detected in bone marrow-derived macrophages in culture | RT-qPCR | [23] | |
| Expressed on culture CD11b+ bone marrow-derived cells that integrated into LV after reintroduction into mice | FACS, IHC | [53] | |
| Detected in activated peritoneal macrophages in vivo and in RAW264.7 macrophages in vitro | RT-qPCR, FACS | [30] | |
| Expressed by monocytes freshly purified from human blood | IHC, RT-PCR | [20] | |
| Podoplanin | Detected on TG-stimulated peritoneal macrophages in culture | FACS, IHC | [16] |
| Co-expressed with F4/80+ cells incorporated into LV in vivo | IHC | [19] | |
| Expressed by myeloid cells incorporated into LV in vivo | IHC | [23] | |
| Expressed on CD11b+ bone marrow-derived cells that integrated into LV in vivo | IHC, FACS | [53] | |
| Co-expressed on CD11b+ cells incorporated into LV in vivo and in activated peritoneal macrophages in vivo | IHC, RT-qPCR | [30] | |
| Expressed by cultured monocytes purified from human blood | IHC, RT-PCR | [20] | |
| LYVE-1 | Co-expressed on CD11b+ cells in LV in vivo and by TG-stimulated peritoneal macrophages in culture | IHC, FACS, IHC | [16] |
| Co-expressed on F4/80+ cells incorporated into LV in vivo | IHC | [19] | |
| Co-expressed on F4/80+ cells incorporated into embryonic LS and LV | IHC | [52] | |
| LYVE-1 | Co-expressed on F4/80+ cells incorporated into LV in vivo | IHC | [23] |
| Expressed on culture CD11b+ bone marrow-derived cells that integrated into LV after reintroduction into mice | FACS, IHC | [53] | |
| Co-expressed on CD11b+ cells incorporated into LV in vivo and inactivated peritoneal macrophages in vivo | IHC, RT-qPCR | [30] | |
| Expressed by monocytes freshly purified from human blood | IHC, RT-PCR | [20] | |
| Prox-1 | Co-expressed on CD11b+ cells in LV in vivo and by TG-stimulated peritoneal macrophages in culture | IHC, FACS, IHC | [16] |
| Co-expressed with F4/80+ cells incorporated into embryonic LS and LV | IHC | [52] | |
| Expressed by myeloid cells incorporated into LV | IHC | [23] | |
| Expressed on cultured CD11b+ bone marrow-derived cells that integrated into LV after reintroduction into mice | FACS | [53] | |
| Co-expressed on CD11b+ cells incorporated into LV in vivo; Activated peritoneal macrophages in vivo expressed reduced levels compared to control group | IHC, RT-qPCR | [30] | |
| Tie2 | Activated peritoneal macrophages in vivo expressed reduced levels compared to control group | RT-qPCR | [30] |
TG, thioglycolate; IHC, immunohistochemistry; LV, lymphatic vessels; FACS, fluorescence-activated cell sorting; LS, lymph sacs.