Table 4.
LECP incorporation into inflamed lymphatic vessels.
| Model | Cell origin or type | Tag | Markers | Time point of analysis | Integration of LECP into LV | Ref. |
|---|---|---|---|---|---|---|
| LPS induced peritonitis (m) | Native macrophages | none | CD11b, F4/80, LYVE-1 | 2 days a | ~50% of LV contained macrophages | [30] |
| LPS induced peritonitis (m) | RAW264.7 macrophages | GFP | CD11b, F4/80, LYVE-1, Podo | 7 days a | ~20% of LV contained macrophages | [30] |
| Corneal micropocket (m) | CD34+/VEGFR-3+ BM-LECP | GFP | LYVE-1 | 1–4 days b | ~1.5% of lymphatic endothelium | [15] |
| Corneal micropocket (m) | CD34+/VEGFR-2+ BM-LECP | GFP | LYVE-1 | 1–4 days b | ~0.5% of lymphatic endothelium | [15] |
| Corneal micropocket (m) | Cultured Podo+ BM-MNC | DiI | LYVE-1 | 7 days b | 5.2% of LV contained DiI+ cells | [53] |
| Skin and ear wound (m) | Cultured Podo+ BM-MNC | DiI | LYVE-1 | 7 days b | 5.5% of LV contained DiI+ cells | [53] |
| Liver of irradiated mice c | Hematopoietic stem cells | GFP | LYVE-1, VEGFR-3 | 1 month b & >1 year b | 2.4% & 3.2% of LV contained GFP+ cells | [217] |
| Gastro-intestinal tissue of irradiated mice | Hematopoietic stem cells | GFP | LYVE-1, VEGFR-3 | >1 year b | 1.0–1.4% of LV contained GFP+ cells | [217] |
| Skin and ear wound (m) | Fresh Podo+ BM-MNC | DiI | LYVE-1 | 7 days b | detected, not quantified | [53] |
| Corneal inflammation (m) | BM-MNC | GFP | CD11b, LYVE-1, Prox-1 | 3 or 7 days a | detected, not quantified | [16] |
| Skin wound (m) | Native myeloid cells | none | F4/80, LYVE-1 | 5 days a | detected, not quantified | [19] |
| Kidney transplant rejection (H) | Presumably BM | none | Y-chromosome, LYVE-1, Podo | N/A | 4.5% of LEC were Y-chromosome+ | [20] |
| Interstitial lung disease (H) | Native macrophages | none | CD68, Podo, VEGFR-3 | N/A | ~1.6 cells/mm of LV | [218] |
| Oncocerciasis (H) | Native macrophages | none | CD68, LYVE-1 | N/A | detected, not quantified | [29] |
LV, lymphatic vessels; (m), mouse; BM bone marrow; BM-MNC, bone marrow mononuclear cells; DiI (1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate), dye used for cell tracking; Podo, podoplanin; (H), human; a time after onset of inflammation; b time after adoptive transfer of progenitor cells; c incorporation was also detected in non-irradiated animals.