Figure 3.
Presynaptic release of glutamate remains intact in stg RTN. Representative continuous sEPSC recordings at positive holding potential (Vh = +40 mV) in WT (A) and stg (B) RTN cells. C, Mean frequency of sEPSCs in stg (n = 14) and WT (n = 18) RTN cells is similar at positive holding potentials (p > 0.05). Furthermore, sEPSC frequency is independent of membrane potential in WT cells (p > 0.05), but in stg cells it is significantly increased by depolarization (p < 0.001). D, Population data showing mean amplitudes of sEPSCs at +40 mV are similar in WT and stg (p > 0.05). Significance assessed with Mann–Whitney test or Wilcoxon's test. **p < 0.005, ***p < 0.0005. ns, Not significant.