Enhancer trap transgenic lines. (A–C) Postmetamorphic juveniles from different Minos-based enhancer trap transgenic lines from the Bioresource Project in Japan (http://marinebio.nbrp.jp/ciona/). (A) GFP expression seen in the endostyle, retropharyngeal band, esophagus, central nervous system, oral siphon, and atrial siphon. (B) GFP expressed in the pyloric gland. (C) GFP is expressed in the central nervous system. Bar, ∼100 µm. All images unpublished, courtesy of Y. Sasakura.