Table 1.
Assessment of Anti-HSV activity ofM. peltatacrude methanolic extract and its constituents by MTT assay
| Test drug | CC50a |
HSV-1 F at MOI: 0.5 |
HSV-2 G at MOI: 0.5 |
||
|---|---|---|---|---|---|
| Antiviral activity (EC50b) | Selectivity index (SI)c | Antiviral activity (EC50b) | Selectivity index (SI)c | ||
| Crude methanolic extract |
452 |
57.5 ± 2.45 |
7.86 |
61.2 ± 3.1 |
7.38 |
| Fraction A |
174 |
7.8 ± 1.6 |
22.3 |
8.2 ± 1.8 |
21.21 |
| Fraction B |
215 |
105.0 ± 5.7 |
2.04 |
120.0 ± 7.2 |
1.79 |
| Ursolic acid (isolated) |
110 |
5.5 ± 0.54 |
20 |
5.8 ± 1.1 |
18.97 |
| Acyclovir | 130 | 2.1 ± 0.1 | 61.9 | 2.9 ± 0.1 | 44.8 |
a The 50% cytotoxic concentration for Vero cells in μg/ml.
b Concentration of compound (μg/ml) producing 50% inhibition of virus induced CPE of three separate experiments.
c Selectivity index (SI) = CC50/EC50.