Table 3.
Real-time PCR primer combinations
| Primer name | Gene ID | Species | Primer | Primer sequence (5’- 3’) |
|---|---|---|---|---|
| GAPDH |
Z19086 |
C. coturnix |
Forward |
GGCACTGTCAAGGCTGAGAAT |
| Reverse |
GCATCTCCCCACTTGATGTTG |
|||
| HMGCR |
NM_204485 |
G. gallus |
Forward |
GCAGAGGGCCTTACAAC |
| Reverse |
GGAGGAGCAAGCCGTAT |
|||
| FDFT1 |
NM_001039294 |
G. gallus |
Forward |
GCCATCATGTACCAGTATGTG GAA |
| Reverse |
GCTGCGTCTTGTTGGAGGAA |
|||
| SQLE |
NM_001030953 |
G. gallus |
Forward |
GAGGTAGAAATTCCTTTTCCAACATCT |
| Reverse |
GCCGTGATGGAAGGACCTT |
|||
| DHCR7 |
XM_420914 |
G. gallus |
Forward |
GGGAAAGATTGGAAACGCTACA |
| Reverse |
CAGATTCTGTGTCAGCCTTAAAACA |
|||
| ABCG5 |
XM_419457 |
G. gallus |
Forward |
ATTACAAGATCCCAAGGTCATGCT |
| Reverse |
GAGACGATCTGGTTTGCAGTCA |
|||
| ABCG8 |
XM_419458 |
G. gallus |
Forward |
GCCTTCCAGCATGTTTTTCAG |
| Reverse |
CGCAACCGTAGCTCTGCTATT |
|||
| APOA1 |
D85133 |
C. coturnix |
Forward |
TCTGGTGCAGGAATTCAAGGA |
| Reverse | TCATCCAGGAGGTCGATCAAG |