Table 2.

MBN-base/MBN-DDT primer information for qPCR (F = forward, R = reverse)

Gene	Primer sequence	Annealing temperature	Amplicon length
CYP6M2	F 5’- CAT GAC ACA AAC CGA CAA GG -3’	60.0°C	235
	R 5’- GGT GAG GAG AGT CGA CGA AG -3’
CYP6AK1	F 5’- TCA TCG AGC GAC AGT GTA CC -3’	58.3°C	251
	R 5’- AAA GTG TGA CCC CAG ACA GG -3’
CYP6P3	F 5’- CGA TTC TTC CTG GAC ATC GT -3’	58.3°C	141
	R 5’- CTT GCC CAA ACT ACC GTC AT -3’
TPX4	F 5’- CAG CTG ACA GAC CGA TTA AG -3’	58.3°C	116
	R 5’- CCG TTC GGG AAC AGT TTG TCT -3’
β-actin	F 5’- ACC AAG AGC CTG AAG CAC -3’	*	123
	R 5’- CGA GCA CGA CAC ACT ATA TAC -3’

* Annealing temperature used was the same as the target gene of interest.