Table 2.
RAD sequence reads of the 38 SNP candidate markers linked to anthracnose disease resistance gene Lanr1 in cultivar Tanjil of Lupinus angustifolius discovered by RAD sequencing on NGS platform Solexa HiSeq2000
| Candidate markers* | Coverage depth** | DNA sequences (5’-3’)*** |
|---|---|---|
| Candidate marker 1 |
7.0/6.4 |
AATTCAGATTCAAGCGTGAAATATTTATACGTAGGAGAAAATGAGAGAAGGGAGGTCTGTGAGAGAAAGAGTA[G/A]AAAGAAAAAAATAAAAAA |
| Candidate marker 2 |
5.7/6.7 |
AATTCAACACACCGCTCATCTCA[A/C]CTACTTTCAAAATCAACCGTCTATGGATTATCTCCACTAAGATAATATATATTATAATAAAAAATGAA |
| Candidate marker 3 |
7.1/7.5 |
AATTCCACAAATTGAAAAACCCGACCGCTTTTTTCATGAAATGCCAATGAAAATG[T/C]TGTTAGTACTAATACTAATTAATTGACTTCTATAAG |
| Candidate marker 4 |
11.9/13.9 |
AATTCCACCAGGATTGCACAACACTAATCTCAACTTGGTCTTGTTGTTTTTCATAATTGGCATCTACACCAATTG[C/G]ATATCAACACTGCTTT |
| Candidate marker 5 |
7.2/7.7 |
AATTCTTCTTCAGAAACAAGGAGCCAATC[G/C]GATTCCAAATGCACTGAAGGAAGCTCAAGCAGAAAGCAAAATCTAAAGAGATTAATCTGATA |
| Candidate marker 6 |
9.0/8.8 |
AATTCTTACAATGAACTTCTATTTTCATTTTGCACTACTAAATCATATTTGCAATATATATGTGTTATATTATGCAAACTGAATCTTT[A/G]TAT |
| Candidate marker 7 |
13.7/13.3 |
AATTCATCTGTACTGATTTCTTTCATATCAAATAGCAGCAGTGGCAATCCTAAAAATAGAATGACCTCT[C/T]GATGTGTGTGCATTATGTGTAT |
| Candidate marker 8 |
8.7/9.1 |
AATTCTTTAAACTTTTGTTCCTTTATTTGAAGTTCTCTTGCTTTTTCTAAATCAAGTAAATTAGGAGTC[A/C]TAACAAAGTTTACCTTAGAGCA |
| Candidate marker 9 |
9.1/8.8 |
AATTCAGAGACAGGACTCATGGCTTATTGGTAATTGGAACATT[C/T]GGAAATAGCAATGAAATCAAAGAAATTCTAGATAACCAAAAAAACACT |
| Candidate marker 10 |
15.4/15.8 |
AATTCTTAAAACAAATTTATACAAGTTTCTCTTTTATTCATTCATGAGAATAAGTCAAAATTGAAAATGGAAGGTAACCC[A/T]TATCAAAGCGT |
| Candidate marker 11 |
4.2/10.9 |
AATTCTATATCTTAGTGATTC[C/A]GTGTAACTTATACTACTAGAGGTAGGTGTGGGATGGCAATTTGACTAGTGAAATGAAGTAAAGTGACTGC |
| Candidate marker 12 |
6.8/8.5 |
AATTCGAACTCATATATGAATACGTGGGCATTCTTTATCAATTGAGTTAGTTGATTATT[A/T]TTTTAATTTCTTAAAAAGTTTTGAGAGCACTT |
| Candidate marker 13 |
10.9/11.4 |
AATTCTGATGTGAAACAACGTGAAAAGAAAGGAGAAAAATCTGTCTTCTGAACAAGAAATGGACA[G/A]ATATCAAAGCTCAGCCAGGAGCATTT |
| Candidate marker 14 |
7.42/9.24 |
AATTCTCTCACAAGCTAATCGACATTCATCTGTATGCTTTGC[T/A]ACAGTAGACTCTAGGACTTTCTCAAGTTCAGTCACTCTATTGCTTAGCT |
| Candidate marker 15 |
12.8/14.2 |
AATTCTCATAATATTTTATAGATCTCATTTAAGAGTTTAAATAGTTAG[G/C]ATAAAGTTTTTTACACATTATTATGATTAGTATTAGTATGTAT |
| Candidate marker 16 |
12.0/11.6 |
AATTCCCCTCAAATTAACACTGTTTCTCGTTTGGGTTCAAGAGCCCTTTGCTTATTGCTTTGAGTTTAAA[G/C]CTCCAAACTTTAAATAGAGTT |
| Candidate marker 17 |
14.9/15.6 |
AATTCCAGAGGATACACATGACACACTACAACATTAGTACCCG[A/G]CAATGCCTCAAAACTGCGGTCTAATATGAAAAAATCGATGTCTTTGTT |
| Candidate marker 18 |
13.3/12.5 |
AATTCACACTCAATCATGTTCTGCAGCTTAAACT[A/G]AAAAAACAATAGGACCTTTTGCTCTTGATAAAATTTCTGATTTAAAAAATGTACAAG |
| Candidate marker 19 |
18.9/19.2 |
AATTCATTCAAGGGTCTTGTCAATCAATTGA[A/C]AAAGATATATGATGAGTTGCAAGCACTTATGCTACTAAGTTCGTTGCTTGAAAACTGGGA |
| Candidate marker 20 |
12.9/13.6 |
AATTCTAGTTTTGTCTTGGTCCTATTGTTTGCTTGATTTTTCAATTCATTACTAAACTATT[C/T]TGACAGTTACTGCATACTATTTGCCTTAAA |
| Candidate marker 21 |
16.2/19.2 |
AATTCTGAAGCAAGTGTATACATTTAAGTTCTAGAAATAGAAAGGATACACTCACGG[G/A]ATGAGATAGCCAAGATAAACTATACATGGAATAT |
| Candidate marker 22 |
28.9/26.1 |
AATTCTAGTTTCTTTTATCTTGTTCTTTTCCCAGAAGATATTACTTGTCTTTAATTTTCTTTTGGGTGGGA[A/G]TGGGAGTGAGGGGAATTAAA |
| Candidate marker 23 |
18.7/19.8 |
AATTCTTTTGTTGATAACCTCAAACAAGATGGCCTAGTGTTAATCATTGGTTAGAACA[C/G]TGAAATTAATTTTTGTTTTTAAGACAACATATA |
| Candidate marker 24 |
20.2/19.8 |
AATTC[T/C]TAATAGGTGTAGTAGGATATATAATAAGAATACTTAAATTACTTAAAAAAGTACATAGATAGATAAATATCACTATTCGACACTCT |
| Candidate marker 25 |
14.4/16.6 |
AATTCTCCGTCTCTCCCCCTTCACCTTGCGGAGCAAAATCCCTCAATAGGTCCCAAGTTGACGAATCATTATCCA[C/T]CGCCGAAATCCTAATT |
| Candidate marker 26 |
7.9/7.1 |
AATTCTCAAGTTAAATATGATGTGGCTAACAGGGTTCACTTCTAGGTCTCGAGGTT[G/A]CTGATGCTGAAAGATCTTATCATACTGAATTGATC |
| Candidate marker 27 |
25.1/27.5 |
AATTCCAAATCTTTAGCTGAAAAGTCAATTAACAACTATCAACATTTTATTCAGTAGAAGTG[C/T]ATAACAGACTAGATATTGGTATTAATAAT |
| Candidate marker 28 |
21.4/20.2 |
AATTCATAACTACTTTGTAATTGATCAAGCTTTTCTTGCTGATCATCATTCTCTTTT[C/T]TCTCTGATTCTCTATGTTGAGTATCAAGAAATAA |
| Candidate marker 29 |
14.3/16.0 |
AATTCAAGCTGCTATCAACTTT[A/G]AAGCTCTCTCCCCACATGCTAACTTGATCAAATGGCTCATATATGCCCATCTTTTTCCCAATGACTAGT |
| Candidate marker 30 |
8.9/11.0 |
AATTCAACAACAAAAAATCATCGTAACGCCAG[T/C]AATCCTCATTGCAACAACTATAATGGCCGCAACCATAATTTAAAATACCGATTTTGTTT |
| Candidate marker 31 |
20.5/21.1 |
AATTCATATTCCACACAGATTTGTTCAACTGTTGAATTTGCTTACATGTCCTGAATCAAAAAGAAGAGAAAAGTTTAGACAC[G/T]ATGCGGTCA |
| Candidate marker 32 |
18.4/17.0 |
AATTCGAAATGGAGAGCAATGTCACTTTCATAAATGGGATAAACAAAATTTCGTTACTTAGTGCAACAGTTGGAATGCC[T/G]GTAATAACAAGC |
| Candidate marker 33 |
31.7/31.2 |
AATTCTGGTGCTGCGACAGAAGTGTTATGCA[A/G]AACTATTAGTCATCCTCTTAAGGTAAAACCTATTATGCTGTTACAAAAGTTCAGCTTGCC |
| Candidate marker 34 |
21.9/17.4 |
AATTCATTACCCTTGACA[A/C]CCTACATGAATTAGTAAAAATAAGTTTAGCCAATTCTAACATGGAACCTGTAGCATATAAAACCAATGTTCTA |
| Candidate marker 35 |
27.2/26.1 |
AATTCAAGCAAATTGGACCATGTGAAATCTGGATACTGTTTTGTCCCATCTTTGACAACAATAGCCTCTGG[T/G]GCTCTCAACACTTTGTCTTC |
| Candidate marker 36 |
26.5/28.8 |
AATTCTGCTAGTGAAGCTGG[A/G]GTTTTTCCTGCACCTGCACATTTTAGACTAGTGCCACATGAACCTGTTTGGCAATTGCCATTGCCACTTTT |
| Candidate marker 37 |
25.8/24.8 |
AATTCTTAGATACTTACAAGGAACAAAATAATTCGGTATATGGTATAAGACCAACACCAACTCAACATTGCACG[G/A]CTACACTAACAGTGATT |
| Candidate marker 38 | 32.2/27.6 | AATTCAGTGGAATATTTCATGTTCACAAACACATTCGACCATAGCGAGAAAGTGCACCTCTC[T/A]TATTCTATTCATACCTGAATGGTTCTAAG |
*Candidate marker numbers were consistent to the candidate makers listed in Table 1.
**The first number was the average coverage depth (x) of the nucleotide from the 10 plants susceptible to anthracnose disease; the second number was the average coverage depth of the nucleotide from the 10 plants resistant to anthracnose.
***The first nucleotide of each SNP in bracket for each RAD sequence read was from parental plant Unicrop; the second nucleotide of the SNP in bracket for each RAD sequence read was from parental plant Tanjil.