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. Author manuscript; available in PMC: 2012 Aug 29.
Published in final edited form as: Nat Neurosci. 2011 Feb 6;14(3):357–365. doi: 10.1038/nn.2753

Figure 2. Chronic partial blockade of cerebellar sodium pumps results in ataxia and dystonic-like postures.

Figure 2

(a) Coronal schematic of the cerebellum showing the regions affected by chronic perfusion of ouabain.

(b) Merge of the bright field picture of a coronal section of the cerebellum with the fluorescence intensity profile obtained after perfusion of 18 ng/h bodipy-FL-ouabain for 18 h.

(c) Estimated concentration of ouabain in the cerebellum as a function of lateral distance from the center of the canula with different concentrations of perfused ouabain.

(d) Dystonic postures in a mouse caused by perfusion of 36 ng/h ouabain into the cerebellum for 18 hours. Arrows point to the commonly observed hyperextensions of anterior and posterior limbs.

(e) Effects of chronic perfusion of 0–72 ng/h ouabain into the cerebellum on locomotor activity in mice at 24, 48 and 72 hours. The decreases in locomotion reflected in the locomotion disability scores were a reflection of the ataxia and dystonia in these mice and not akinesia and rigidity as seen when ouabain was perfused into the basal ganglia. mean±s.e.m.

(f) Severity of ouabain-induced dystonia in mice chronically perfused with 0–72 ng/h ouabain into the cerebellum at 4, 12, 24, 48 and 72h. mean±s.e.m.

(g) Symptoms associated with chronic perfusion of ouabain into the cerebellum.