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. 2012 Aug 29;7(8):e44005. doi: 10.1371/journal.pone.0044005

Figure 10. BnPHR1 directly regulating ATPT2 encoding high-affinity Pi transporter in transgenic Arabidopsis.

Figure 10

(A) The partial sequence of ATPT2 promoter containing two overlapped P1BS cis-elements (GNATATNC) (uppercase and underlined). (B) Western blotting assay of 35:GFP and 35:BnPHR1-GFP transgenic Arabidopsis with GFP antibody. (C–D) ChIP-qPCR analysis of the ATPT2 promoter sequence. ChIP assay was performed with chromatin prepared from 35:GFP and 35:BnPHR1-GFP transgenic Arabidopsis roots. Genomic DNA fragments that coimmunoprecipitated with GFP antibody were analyzed by real time qPCR using primers amplifying ATPT2 promoter fragment (C) and ATPT2 coding fragment (D) respectively. The experiments were repeated three times, and three replicates were included for each sample in each experiment. The data are presented as means standard deviation (n = 3).