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. 2012 Aug 29;7(8):e43845. doi: 10.1371/journal.pone.0043845

Figure 2. Binding domains recognized by neutralizing mAbs.

Figure 2

Plates were coated with GT1b (A) or recombinant syt II-LD (B) respectively. HRP-conjugated BoNT/B Hc was pre-incubated with neutralizing mAbs at molar ratio of 1∶100. Bound BoNT/B Hc was detected by adding TMB as a substrate.