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. 2012 Aug 29;7(8):e43845. doi: 10.1371/journal.pone.0043845

Figure 3. Reactivity of synthetic epitope peptides with neutralizing mAbs.

Figure 3

Binding of synthetic peptide with mAb. Peptides P1, P2, P3, containing the motifs recognized by 8E10, 5G10 and 2F4 respectively, were synthesized and conjugated with KLH, which were indicated as P1-, P2-, P3-KLH. The binding abilities of mAbs were detected by ELISA. A non-associated peptide was set as control peptide (CP), and conjugated with KLH as CP-KLH. (B) Competitive inhibition of BoNT/B Hc binding with mAbs by synthetic peptides. CP-KLH: synthetic peptide THPHLPRALMRS was coupled to KLH (bearer protein) to generate CP-KLH; P1-KLH: synthetic peptide YFCISKWYLKEV was coupled to KLH (bearer protein) to generate P1-KLH. P2-KLH: synthetic peptide PISDSDEFYNTI was coupled to KLH (bearer protein) to generate P2-KLH; P3-KLH: synthetic peptide IKLKKDSPVGEI was coupled to KLH(bearer protein) to generate P3-KLH.