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. 2012 Aug 29;7(8):e43845. doi: 10.1371/journal.pone.0043845

Figure 6. Cross-reactive and cross-neutralizing effects of 8E10.

Figure 6

(A) Amino acid sequence alignment of the carboxyl-terminal region of BoNT (A, B, E, F). The residues, which are conservative among BoNTs/A, B, E and F are presented as bold letters. (B) ELISA of 8E10 to BoNT Hcs of different serotypes A, B, E and F. (C) BoNT Hcs (A, B, E, F) were separated by SDS-PAGE under reducing conditions and visualized by western blotting with 8E10. (D) Cross-neutralizing effects in vivo. 100 µg of 8E10 was pre-incubated with 20 LD50s of BoNT/A, B, E, F for 1 hour respectively. The mixtures were injected into mice, and the final death tally was determined 4 days after injection.