Figure 6. Mitochondrial metabolism dominates the short-wavelength components of the IOS. A.

) Inducing HSD in the presence of 10 µM CN⁻ did not cause marked alterations in the multispectral IOS or the averaged reflectance profiles. Only the 440 nm nadir was less pronounced and the posthypoxic secondary reflectance increase was prolonged. B) When HSD was induced directly by 1 mM CN⁻ (in the presence of O₂) especially the reflectance increase at short wavelengths was intensified and the nadir at 440 nm was abolished; reflectance at longer wavelengths increased less intensely. Note that a recovery of the reflectance increase at short-wavelengths did not occur, and that a posthypoxic secondary reflectance increase at longer wavelengths was absent. C) Inducing HSD in the presence of FCCP (10 µM) caused a less intense reflectance increase especially at intermediate and longer wavelengths. Also the secondary reflectance increase during the posthypoxic recovery tended to become more intense and prolonged.