Figure 8. Determination of amino acids responsible for Al transport activity of HmVALT1 or HmPALT1.

(A) Al tolerance assay of amino acid replaced HmVALT transformant. Δhsp150 yeast cells that harbored HmVALT-L162T and −G196A were spotted onto LPP medium (pH 3.5) with or without 2 mM Al₂(SO₄)₃, and the plates were incubated at 30°C for 4 d.HmVALT-L162T transformant grew less indicating to become more Al-sensitive but that of HmVALT-G196A grew at similar level. (B) Al tolerance assay of amino acid replaced HmPALT transformant. Wild type yeast cells that harbored HmPALT1-T114G, −E181Δ and −H188P were spotted onto LPP medium (pH 3.5) with or without 1 mM Al₂(SO₄)₃, and the plates were incubated at 30°C for 4 d. HmPALT1-E181Δ transformant was less sensitive to Al, but others showed to be more sensitive.