Table 1. Incorporation of increased SELEX cycle number improves peak discrimination.
Number of cycles useda | Total peaks calledb | Number of “Best” peaksc | Percent “Best” peaks of total |
3 | 2,628 | 457 | 17.4 |
2 | 4,255 | 1,350 | 31.7 |
1 | 7,274 | 7,274 | 100 |
“Number of cycles used” does not include control.
“Total peaks called” refers to peaks called using TFAST's algorithm.
“Best” peaks are peaks with the highest weight scores for that set, as calculated by TFAST.