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. 2012 Aug 29;7(8):e44283. doi: 10.1371/journal.pone.0044283

Figure 1. The general reaction scheme for N-ethylmaleimide on biological thiols.

Figure 1

The product formed is an N-ethylsuccinimido conjugate and the monoisotopic mass of the reactant is increased by 125.048. For example the glutathione (m/z 308.0911) conjugate is N-ethylsuccinimido-S-glutathione (m/z 433.1391).