Figure 1. The general reaction scheme for N-ethylmaleimide on biological thiols.
The product formed is an N-ethylsuccinimido conjugate and the monoisotopic mass of the reactant is increased by 125.048. For example the glutathione (m/z 308.0911) conjugate is N-ethylsuccinimido-S-glutathione (m/z 433.1391).
