Table 1. Metabolites (attomol/cell) extracted from samples taken during respiratory oscillation (minimum dissolved oxygen; figure 4), detected by CE-MS and the methods used to extract them. nd – not detected.

Name	Sonication	Sonication -Q 1	Freeze thaw	BB 2, 3	BB 2 -NEM 4 before 5	BB 2 -NEM 4 after 6	BB 2 -NEM 4 -Q 1
Pyruvate	2.170±0.876	2.219±0.132	1.443±0.612	1.273	1.482±0.175	1.666±0.039	0.604±0.029
Lactate	1.653±1.187	2.495±0.069	0.381±0.124	1.339	0.949±0.064	1.261±0.107	1.437±0.088
Fumarate	1.607±0.617	2.180±0.144	1.096±0.657	0.683	0.629±0.040	0.626±0.061	0.473±0.002
2-Oxoisopentanoate	nd	nd	nd	0.145	0.178±0.009	0.154±0.020	0.091±0.006
Succinate	1.451±0.306	5.073±0.356	1.680±0.423	1.373	1.137±0.036	1.709±0.262	8.124±0.172
Malate	2.231±1.592	6.874±0.560	2.150±1.509	2.585	2.292±0.074	2.221±0.151	2.036±0.036
2-Oxoglutarate	nd	nd	nd	0.948	0.974±0.160	nd	nd
PEP	nd	nd	nd	0.220	0.429±0.079	0.363±0.002	0.193±0.017
DHAP	nd	0.984±0.210	nd	0.230	0.520±0.201	0.526±0.134	0.377±0.083
Glycerophosphate	1.269±0.275	2.175±0.321	1.390±0.989	0.629	0.613±0.021	0.672±0.125	0.719±0.062
cis-Aconitate	0.105±0.056	nd	0.141±0.068	0.521	0.365±0.007	nd	nd
3PG	3.825±2.763	nd	0.873±1.010	4.432	4.317±0.343	4.969±0.225	0.703±0.045
Ru5P	0.264±0.113	nd	nd	0.254	0.473±0.120	0.453±0.040	0.172±0.038
G1P	0.757±0.353	0.699±0.030	0.011±0.004	0.504	0.539±0.060	0.656±0.119	0.631±0.020
F6P	0.498±0.232	0.716±0.028	nd	nd	0.451±0.048	nd	nd
G6P	0.641±0.873	2.399±0.110	0.010±0.003	5.110	7.325±0.247	5.432±0.333	1.661±0.046
2,3-DPG	nd	nd	nd	0.032	0.045±0.011	nd	0.012±0.017
6-Phosphogluconate	0.441±0.246	0.299±0.047	nd	0.889	1.165±0.350	0.933±0.066	0.122±0.019
S7P	0.817±0.431	0.386±0.011	nd	0.843	0.951±0.035	0.677±0.070	0.103±0.002
F1,6P	1.160±0.810	7.337±1.831	0.011±0.004	0.547	0.908±0.033	0.730±0.172	0.797±0.064
Citrate	2.470±0.457	3.825±0.270	1.035±1.119	2.174	2.316±0.153	2.040±0.250	1.650±0.037
CMP	0.217±0.040	0.223±0.024	0.106±0.066	0.178	0.123±0.007	0.211±0.042	0.620±0.010
AMP	3.668±0.599	2.264±0.222	1.760±1.211	2.239	1.278±0.099	2.505±0.562	6.824±0.225
IMP	0.540±0.086	0.483±0.035	0.268±0.168	nd	0.083±0.017	nd	nd
GMP	0.475±0.081	0.292±0.037	0.171±0.151	0.233	0.155±0.014	0.279±0.071	1.576±0.004
NADPH	0.003	0.280±0.010	nd	nd	nd	nd	0.198±0.002
CoA	0.094±0.027	0.125±0.008	0.018±0.023	0.020	0.160±0.017	0.139±0.036	0.228±0.014
dTDP	0.014±0.002	0.016±0.002	nd	0.046	0.035±0.004	0.041	0.039±0.001
CDP	0.135±0.017	0.151±0.027	0.034±0.044	0.297	0.235±0.019	0.315±0.009	0.221±0.004
Acetyl CoA	0.025±0.002	nd	0.010±0.012	0.068	0.095±0.005	0.085±0.014	0.084±0.004
ADP	1.918±0.274	1.979±0.326	0.506±0.699	3.597	3.110±0.080	3.716±0.333	3.309±0.025
GDP	0.264±0.004	0.273±0.052	nd	0.358	0.320±0.020	0.400±0.035	0.712±0.012
dCTP	nd	nd	nd	0.034	0.039±0.006	0.035±0.004	nd
dTTP	0.006±0.001	0.012±0.002	nd	0.062	0.076±0.004	0.063	0.026
CTP	0.054±0.008	0.127±0.046	nd	0.601	0.739±0.009	0.569±0.030	0.151±0.005
UTP	0.262±0.027	0.216±0.047	0.074±0.102	1.635	1.939±0.065	1.509±0.027	0.550±0.008
dATP	0.005±0.001	0.007±0.001	nd	0.051	0.058±0.012	0.046±0.010	0.026±0.006
ATP	1.167±0.218	2.271±0.490	0.361±0.506	8.814	10.520±0.588	7.605±0.088	3.082±0.037
GTP	0.165±0.016	0.240±0.071	nd	1.774	2.027±0.112	1.654±0.123	1.037±0.008
NAD+	2.120±0.172	1.707±0.086	nd	3.504	3.711±0.107	3.639±0.013	3.358±0.082
NADH	0.025±0.003	2.421±0.313	nd	nd	nd	nd	0.964±0.149
NADP+	0.306±0.023	0.183±0.013	0.068±0.093	0.520	0.520±0.013	0.504±0.032	0.382±0.011
FAD	0.052±0.007	0.072±0.007	nd	nd	0.184±0.012	0.144±0.087	0.087±0.027
Gly	1.938±0.171	2.006±0.120	1.895±0.056	7.184	7.270±0.745	8.590±0.027	16.670±5.088
Ala	2.018±0.080	2.012±0.031	2.060±0.054	190.603	198.371±16.626	228.475±3.048	302.253±0.606
GABA	2.000±0.085	1.975±0.198	1.974±0.106	1.021	1.097±0.113	1.259±0.068	1.377±0.037
2AB	2.016±0.155	2.020±0.132	2.047±0.186	0.893	0.976±0.071	1.113±0.052	1.310±0.075
Ser	2.113±0.178	2.163±0.058	2.164±0.079	6.481	6.712±0.547	7.691±0.059	10.683±5.029
Pro	2.043±0.228	2.053±0.240	1.985±0.320	5.271	5.512±0.461	6.283±0.038	9.575±0.759
Val	1.871±0.315	2.087±0.123	2.059±0.097	38.476	43.287±3.415	48.741±1.008	55.543±1.200
Homoserine	2.362±1.049	1.714±0.121	1.742±0.134	1.691	1.971±0.168	2.127±0.082	1.410±0.042
Thr	2.500±1.057	2.567±0.899	2.854±1.111	12.228	13.525±1.159	14.918±0.192	16.956±0.692
Cys	nd	nd	nd	nd	0.310±0.049	0.343±0.022	0.444±0.008
Ile	2.042±0.119	2.045±0.162	2.072±0.032	4.053	4.592±0.456	5.340±0.084	6.518±0.618
Leu	2.103±0.143	2.139±0.197	2.112±0.228	1.567	1.589±0.114	1.913±0.045	3.025±0.766
Asn	2.009±0.124	2.034±0.095	2.050±0.134	3.447	3.920±0.304	4.161±0.124	5.038±0.168
Ornithine	1.924±0.087	1.933±0.071	1.865±0.061	9.902	8.199±0.726	8.850±0.796	10.901±2.848
Asp	2.004±0.090	1.955±0.121	1.969±0.050	13.221	55.773±1.133	56.621±0.508	55.545±2.242
Homocysteine	nd	nd	nd	0.132	0.709±0.097	0.737±0.006	0.795±0.036
Adenine	nd	nd	nd	0.151	0.172±0.007	0.155±0.007	0.139±0.003
Gln	2.008±0.113	2.046±0.143	2.013±0.155	104.444	223.521±10.435	228.527±0.257	225.379±0.076
Lys	2.003±0.136	2.009±0.095	2.036±0.144	12.354	34.010±1.118	35.580±0.021	35.572±0.776
Glu	1.980±0.129	1.977±0.115	1.997±0.121	835.237	864.465±19.172	887.669±10.922	886.352±2.001
Met	nd	nd	nd	0.454	0.487±0.061	0.554±0.012	1.003±0.033
Guanine	nd	nd	nd	0.068	0.071±0.001	0.066±0.006	nd
His	1.948±0.094	1.980±0.060	1.944±0.069	12.022	13.150±1.267	14.154±0.263	14.336±1.289
Phe	2.038±0.099	2.006±0.090	2.012±0.045	1.113	1.254±0.073	1.330±0.014	1.710±0.387
Arg	2.020±0.107	1.964±0.065	2.002±0.080	93.775	218.231±10.111	225.316±4.592	214.317±2.327
Citrulline	2.034±0.084	2.007±0.082	2.022±0.083	7.458	8.877±0.960	9.170±0.372	8.951±0.139
Tyr	2.114±1.093	2.076±0.968	2.374±1.191	1.227	1.400±0.090	1.509±0.017	1.843±0.584
SAH	1.932±0.257	1.910±0.223	1.987±0.283	0.318	0.330±0.020	0.407±0.002	0.696±0.007
SAM+	nd	nd	nd	0.530	0.549±0.028	0.553±0.079	0.532±0.008
Trp	2.060±0.099	2.053±0.069	2.058±0.124	0.254	0.287±0.015	0.287±0.028	0.365±0.073
Cystathionine	1.981±0.119	1.957±0.119	1.976±0.135	5.837	7.127±0.603	7.281±0.039	6.559±0.062
γ-Glu-2AB	nd	nd	nd	0.255	0.282±0.026	0.313±0.001	0.279±0.004
γ-Glu-Cys	nd	nd	nd	1.436	7.796±0.500	8.822±0.415	8.220±0.438
Adenosine	2.009±0.152	1.970±0.124	1.949±0.190	0.281	0.444±0.064	0.421±0.156	0.408±0.016
Guanosine	nd	nd	nd	0.108	0.125±0.005	0.131±0.022	0.090±0.008
Glutathione(ox)	2.103±0.279	2.078±0.243	2.162±0.308	13.105	14.840±0.114	15.060±0.199	14.559±0.086
Glutathione(red)	2.004±0.148	2.015±0.150	1.923±0.125	36.580	143.608±12.226	147.043±5.378	129.318±1.290

¹-Q not quenched.

²BB bead-beating.

³All other samples were performed in triplicate, except this one.

⁴NEM N-ethylmaleimide.

⁵NEM added pre bead-beating.

⁶NEM added post bead-beating.