Table 1. Detection of harbor porpoise DNA using qPCR at a controlled site (Fjord&Belt pen) and at natural sites.

	Acoustic detection	Genetic detection
Location	% Porpoise positive days	Positive PCRs	Cycle threshold
Positive control (DNA extracted from skin)		3/3	18, 18, 18
Fjord&Bælt pen		3/3	34, 35, 35
<10 m from F&B pen		1/3	49
>10 m from F&B pen		0/3	-
Site 1	94	1/3	49
Site 2	42	0/3	-
Site 3	63	0/3	-
Site 4	6	0/3	-
Site 5	0	0/3	-
Site 6	0	0/3	-
Site 7	0	2/3	38*, 50*
Site 8	79	0/3	-

Genetic detections at the eight natural sites are compared with acoustic detection rates based on data from static acoustic monitoring devices over the three months prior to eDNA sampling.

^*sequencing of PCR clones indicates these were genetic detections of long-finned pilot whale and not genetic detection of harbor porpoise at this site.