Fig. 4.

Identification of differentiation potential of human ESCs in vitro and in vivo (H-TJ6). a EBs in suspension culture at day 4. Bar: 100 μm. b RT-PCR analysis of the expression of specific markers for the three embryonic germ layers in EBs, including AFP (endoderm), NEUOD-1 (ectoderm) and HBZ (mesoderm). c, d Histopathological analysis of teratomas resulting from in vivo differentiation of human ESCs, including hair follicles (ectoderm) (c), cartilage tissue (mesoderm) (c) and intestinal epithelium (endoderm) (d)