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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Biosyst. 2012 Oct;8(10):2484–2493. doi: 10.1039/c2mb25122a

Figure 5.

Figure 5

Hydroxyl group-targeted method to generate natural product analogs for protein target identification. A. A rhodium catalyzed reaction between an alcohol and a diazoester-functionalized coupling partner results in incorporation of an alkyne moiety. B. FK506 was used as a model natural product to demonstrate the utility of the devised strategy. Following functionalization of FK506 with an alkyne handle, this probe was incubated with RKO cells to facilitate the identification of the binding partner(s) of the natural product probe. Probe design dramatically altered the results of these experiments.