Diversity of amino acid biosynthetic capabilities across all sequenced organisms from the Integrated Microbial Genomes (IMG) database71, separated based on the three domains (Bacteria, red, top panel; Archaea, blue, middle panel; Eukarya, orange, bottom panel). (a.) Predicted frequencies at which species have the ability to synthesize zero to all 20 standard amino acids. (b.) For each amino acid, frequencies at which complete biosynthetic pathways are found across each domain are shown in solid colored bars (Bacteria, red, top panel; Archaea, blue, middle panel; Eukarya, orange, bottom panel). White bars indicate fractions in each domain where one or more biosynthetic gene is missing. Gray bars indicate unknown annotations.