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. Author manuscript; available in PMC: 2013 Nov 18.
Published in final edited form as: J Proteome Res. 2012 May 18;11(6):3090–3100. doi: 10.1021/pr201068b

Figure 4. Comparisons of hpRP-HPLC separations after pooling peptides into differing numbers of fractions.

Figure 4

A) The number of unique peptides identified after pooling separated peptides into 12, 20 or 40 fractions. B) The number of unique proteins identified in each proteome. C) Overlap of proteins identified by at least two peptides in the duplicate 12-fraction analyses. Overlap was assessed based upon exact matches of database entries.